Isolation and identification of proteolytic, amylolytic, lipolytic, and chitinolytic bacteria from shrimp waste

Susana Sirvas Cornejo; Vanesa Buleje; Licia Salvatierra; Michael L. Jaramillo

doi:10.15381/rpb.v28i1.18353

Isolation and identification of proteolytic, amylolytic, lipolytic, and chitinolytic bacteria from shrimp waste

Authors

Susana Sirvas Cornejo Instituto Tecnológico de la Producción (ITP), Lima, Perú. https://orcid.org/0000-0002-8095-7499
Vanesa Buleje Instituto Tecnológico de la Producción (ITP), Lima, Perú. https://orcid.org/0000-0002-8030-9643
Licia Salvatierra Instituto Tecnológico de la Producción (ITP), Lima, Perú. https://orcid.org/0000-0001-6865-2142
Michael L. Jaramillo Instituto Tecnológico de la Producción (ITP), Lima, Perú. https://orcid.org/0000-0001-5556-8793

DOI:

https://doi.org/10.15381/rpb.v28i1.18353

Keywords:

bacterial enzymes, proteases, lipases, amylases, chitinases

Abstract

Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitinolytic capacity on agar plates with specific substrates. The genera Bacillus, Burkholderia, Ochrobactrum, Vibrio, Pseudomonas and Shewanella were identified. Bacteria with enzymatic capacities isolated in the present study, could be used to obtain by-products from shrimp waste as well as other industrial applications.

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Published

02/24/2021

Issue

Vol. 28 No. 1 (2021)

Section

Articles

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How to Cite

Sirvas Cornejo, Susana, Vanesa Buleje, Licia Salvatierra, and Michael L. Jaramillo. 2021. “Isolation and Identification of Proteolytic, Amylolytic, Lipolytic, and Chitinolytic Bacteria from Shrimp Waste”. Revista Peruana De Biología 28 (1): e18353. https://doi.org/10.15381/rpb.v28i1.18353.