Purification and partial characterization of a toxin (Hm3) from Hadruroides mauryi (Francke y Soleglad, 1980) (Scorpiones, Iuridae) venom
DOI:
https://doi.org/10.15381/rpb.v11i2.2451Keywords:
Toxin, Hadruroides mauryi, scorpion, scorpion venomAbstract
A toxin (Hm3) was purified from the scorpion venom Hadruroides mauryi, by ion-exchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7. The toxin produce contraction and immobilize the inoculated limb of white mice, and is a basic protein with a molecular weight of 4,5 kDa. After of 45 minutes inoculated in gastrocnemius muscle of white mice, Hm3 (30,4 µg) increases the plasmatic level of creatine kinase from 252,6 UI/L to 3779,3 UI/L and lactate deshidrogenase from 142,7 UI/L to 248,2 UI/L; likewise increases the level of intramuscular calcium from 34,1 nmoles to 69,3 nmoles. This toxin have not proteolitic or phospholipase activity.Downloads
Downloads
Published
Issue
Section
License
Copyright (c) 2004 Luz Velásquez, Enrique Escobar
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
AUTHORS RETAIN THEIR RIGHTS:
a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.
b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista Peruana de Biologia.
c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating its initial publication in the Revista Peruana de Biologia (the originator of the work, journal, volume, number and date).