Rapid diagnosis and identification by PCR of Yersinia ruckeri isolated of Oncorhynchus mykiss from Canta, Lima, Peru

Authors

  • Susana Sirvas-Cornejo Laboratorio de Biología Molecular, Departamento Académico de Acuicultura, Facultad de Oceanografía, Pesquería y Ciencias Alimentarias; Universidad Nacional Federico Villarreal. Calle Roma 350, Miraflores, Lima 18, Perú.
  • Claudia Cecilia Sánchez-Robinet Laboratorio de Biología Molecular, Departamento Académico de Acuicultura, Facultad de Oceanografía, Pesquería y Ciencias Alimentarias; Universidad Nacional Federico Villarreal. Calle Roma 350, Miraflores, Lima 18, Perú.
  • César Peña-Domínguez Laboratorio de Biología Molecular, Departamento Académico de Acuicultura, Facultad de Oceanografía, Pesquería y Ciencias Alimentarias; Universidad Nacional Federico Villarreal. Calle Roma 350, Miraflores, Lima 18, Perú.

DOI:

https://doi.org/10.15381/rpb.v18i3.451

Keywords:

Yersinia ruckeri, Oncorhynchus mykiss, PCR, diagnosis, Enteric Red Mouth Disease.

Abstract

Twenty individuals of rainbow trout were sampled (fry and juveniles) from Acochinchan Fishfarm (Canta, Lima - Peru), and analyzed with the Polimerase Chain Reaction test (PCR ) in order to achieve a rapid identification of Yersinia ruckeri, which is the pathogen agent that causes the enteric red mouth disease (ERM) and produces high rates of mortality. Nine fish samples were asymptomatic, while 11 of them showed signs of ERM. In addition, 22 bacterial strains were isolated from the liver, spleen and kidney. PCR and specific primers (16S rRNA), were used to amplified a specific 575 bp DNA fragment of Yersinia ruckeri. Nineteen strains were identified as Yersinia ruckeri by PCR in symptomatic and asymptomatic fishes. It was established a diagnosis time of 26 hours, compared with the 2 or 3 days that would take the diagnosis using biochemical tests.

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Published

12/30/2011

Issue

Vol. 18 No. 3 (2011)

Section

Articles

License

Copyright (c) 2011 Susana Sirvas-Cornejo, Claudia Cecilia Sánchez-Robinet, César Peña-Domínguez

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How to Cite

Sirvas-Cornejo, Susana, Claudia Cecilia Sánchez-Robinet, and César Peña-Domínguez. 2011. “Rapid Diagnosis and Identification by PCR of Yersinia Ruckeri Isolated of Oncorhynchus Mykiss from Canta, Lima, Peru”. Revista Peruana De Biología 18 (3): 349-53. https://doi.org/10.15381/rpb.v18i3.451.