Agro-transformation and evaluation of resistance to Phytophthora infestansin Solanum tuberosumL. variety Désirée

Authors

  • Jeanette Orbegozo Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • María Lupe Román Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • Cristina Rivera Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • José Carlos Tovar Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • Willmer Pérez Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • Soledad Gamboa Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • Greg Forbes Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • Jan Kreuze Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.
  • Marc Ghislain Laboratorio de Biotecnología Aplicada, Centro Internacional de la Papa (CIP), Apartado 1558, Lima 12, Perú.

DOI:

https://doi.org/10.15381/rpb.v20i3.5202

Keywords:

potato, Phytophthora infestans, Agrobacterium tumefaciens, Rpi-blb2gene.

Abstract

The Oomycete Phytophthora infestans (Mont.) de Bary, the causal agent of the disease known as late blight, is primarily responsible for the decreased in production performance and potato crops worldwide. The integration of the complete Rgenes sequences in the potato genome using Agro-transformation appears an alternative to be considered in the fight against this pathogen. The Rpi-blb2 gene (Rgene) from the wild species Solanum bulbocastanumDunal shows a broad resistance to isolates ofP. infestans,making it an important candidate for plant breeding studies. This paper reports the integration of the Rpi-blb2gene into potato var. Désirée genome by Agrobacterium tumefaciens- mediated transformation system, the molecular characterization of 29 events transformed and whole plant infection with isolate POX67 of P. infestansfrom Peru. Désirée events [Rpi-blb2] 4 and Désirée [Rpi-blb2] 30, showed a substantial resistance to P. infestansinfection confirming complete transfer of the Rpi-blb2gene from a wild species to a cultivated species by genetic transformation.

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Published

12/30/2013

Issue

Vol. 20 No. 3 (2013)

Section

Articles

License

Copyright (c) 2013 Jeanette Orbegozo, María Lupe Román, Cristina Rivera, José Carlos Tovar, Willmer Pérez, Soledad Gamboa, Greg Forbes, Jan Kreuze, Marc Ghislain

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How to Cite

Orbegozo, Jeanette, María Lupe Román, Cristina Rivera, José Carlos Tovar, Willmer Pérez, Soledad Gamboa, Greg Forbes, Jan Kreuze, and Marc Ghislain. 2013. “Agro-Transformation and Evaluation of Resistance to Phytophthora Infestansin Solanum TuberosumL. Variety Désirée”. Revista Peruana De Biología 20 (3): 205-10. https://doi.org/10.15381/rpb.v20i3.5202.