Antimicrobial effectiveness of Lysol® and quaternary ammonium during evaluation of three critical areas of the dental chair
DOI:
https://doi.org/10.15381/os.v27i2.26319Keywords:
Quaternary Ammonium Compounds, Disinfectants, EthanolAbstract
Objective. Determine the antimicrobial efficacy of Lysol® and quaternary ammonium during the evaluation of three critical areas of the dental unit (control tray/buttons, dental chair back, and triple syringe handle). Methods. Through a cross-sectional comparative experimental study, a total of 8 dental chairs were analyzed: three were disinfected with Lysol®, three with quaternary ammonium, and two armchairs were selected as controls (positive and negative). All dental chairs were sampled twice a day: before use and at the end of use, for three days. Sampling was carried out at the Hemisferios University Clinics before and after the application of the disinfectant. The samples were transported in thioglycollate broth for analysis. 1:2 and 1:10 dilutions of each sample were plated on Plate Count Agar and incubated for 24 hours at 37°C. Subsequently, duplicate colony counts were performed, analyzing a total of 864 samples. Results. Lysol® and 5% quaternary ammonium showed equal antimicrobial effectiveness against the three areas analyzed (p>0.05). The area with the highest number of microorganisms was the tray (including control buttons). Conclusion. It was determined that both Lysol® and 5% quaternary ammonium are highly effective in eliminating microorganisms present on the critical surfaces of the dental unit. Furthermore, the tray, including the control buttons, was found to be the most contaminated area before treatment.
Downloads
References
Warthon J, Olarte A, Warthon B. Estimación de la trayectoria de coronavirus covid-19 adheridas a gotitas respiratorias proyectados horizontalmente, considerando la altitud geográfica. Physics 2021:1–9. DOI: https://doi.org/10.48550/arXiv.2101.01359
Tura F, Alves CF dos S, Kirsten VR, Amaral CF do, Dotto PP, Santos RCV. Avaliação da contaminação interna em canetas de alta rotação na prática clínica. Braz Dent Sci. 2011;14(3/4):18-26. DOI: https://doi.org/10.14295/bds.2011.v14i3/4.749
Bustamante Andrade MF, Herrera Machuca J, Ferreira Adam R, Riquelme Sanchez D. Contaminación Bacteriana Generada por Aerosoles en Ambiente Odontológico. Int J Odontostomat. 2014;8(1):99-105. DOI: https://doi.org/10.4067/S0718-381X2014000100013
Palacios Cruz M, Santos E, Velázquez Cervantes MA, León Juárez M. COVID-19, a worldwide public health emergency. Rev Clin Esp. 2021;221(1):55–61. DOI: https://doi.org/10.1016/j.rce.2020.03.001
Bracher L, Kulik EM, Waltimo T, Türp JC. Surface microbial contamination in a dental department. A 10-year retrospective analysis. Swiss Dent J. 2019;129(1):14–21. DOI: https://doi.org/10.61872/sdj-2019-01-01
Featherstone JDB. Dental restorative materials containing quaternary ammonium compounds have sustained antibacterial action. JADA. 2022;153(12):1114–20. DOI: https://doi.org/10.1016/j.adaj.2022.09.006
Coasaca AR, Mendizába MR, Cadillo EM, Nieto ML, Guzmán JN de. Prevalencia de portadores asintomáticos de streptococcus pyogenes y streptococcus pneumoniae en estudiantes del primer año de la Facultad de odontología de la USMP. Rev Kiru. 2009;6(2):84-7.
Redd JT, Baumbach J, Kohn W, Nainan O, Khristova M, Williams I. Patient-to-patient transmission of hepatitis B virus associated with oral surgery. J Infectious Dis. 2007;195(9):1311-4. DOI: https://doi.org/10.1086/513435
Radcliffe RA, Bixler D, Moorman A, Hogan VA, Greenfield VS, Gaviria DM, et al. Hepatitis B virus transmissions associated with a portable dental clinic, West Virginia, 2009. J Am Dent Assoc. 2013;144(10):1110-8. DOI: https://doi.org/10.14219/jada.archive.2013.0029
Cleveland JL, Gray SK, Harte JA, Robison VA, Moorman AC, Gooch BF. Transmission of blood-borne pathogens in US dental health care settings. J Am Dent Assoc. 2016;147(9):729-38. DOI: https://doi.org/10.1016/j.adaj.2016.03.020
Kramer A, Schwebke I, Kampf G. How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect Dis. 2006;6:1–8. DOI: https://doi.org/10.1186/1471-2334-6-130
Pineda Higuita SE, Posada López GA, Giraldo Quintero L, Pulgarín Bedoya L. Resistencia a antibióticos del Staphylococcus aureus en estudiantes de una facultad de odontología. Rev habanera cienc méd. 2020;19(6):e2931. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1729-519X2020000700007%0Ahttp://www.revhabanera.sld.cu/index.php/rhab/article/view/2931/274
Murray CJ, Ikuta KS, Sharara F, Swetschinski L, Robles Aguilar G, Gray A, et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. The Lancet. 2022;399(10325):629–55. DOI: https://doi.org/10.1016/S0140-6736(21)02724-0
Espona Quer M, Salas Sánchez E. Recomendaciones sobre el uso de desinfectantes en el ámbito sanitario. Butlletí d’informació terapèutica del Departament de Salut de la Generalitat de Catalunya. 2013;24(1):1-6.
Torres ARL, Moya JV, Buchelli AEI. Efectividad de Lysol y Glutaraldehído al 2% en piezas de mano de alta velocidad después de ser sometidas a limpieza mecánica. Odontologia. 2019;21(1):34-44.
Tsiaprazi-Stamou A, Monfort IY, Romani AM, Bakalis S, Gkatzionis K. The synergistic effect of enzymatic detergents on biofilm cleaning from different surfaces. Biofouling. 2019;35(8):883–99. DOI: https://doi.org/10.1080/08927014.2019.1666108
Mupparapu M, Kothari KRM. Review of surface disinfection protocols in dentistry: a 2019 update. Quintessence Int (Berl). 2019;50(1):58–65. DOI: https://doi.org/10.3290/j.qi.a41337
Fulford MR, Stankiewicz NR. Dental Disinfection and Environmental Decontamination. In: Infection Control in Primary Dental Care [Internet]. 1st ed. Springer Cham; 2020. p. 105–15. DOI: https://doi.org/10.1007/978-3-030-16307-5_12
Scarano A, Inchingolo F, Lorusso F. Environmental disinfection of a dental clinic during the Covid-19 pandemic: A narrative insight. Biomed Res Int. 2020;2020:1-15. DOI: https://doi.org/10.1155/2020/8896812
Guerreros VMH. Evaluación de la contaminación cruzada en las unidades dentales de la clínica odontológica de la facultad de odontología de la UNDAC 2019 [Internet] [Tesis de Pregrado]. Universidad Nacional Daniel Alcides Carrión; 2020. Disponible en: http://repositorio.undac.edu.pe/bitstream/undac/1857/1/T026_70411514_T.pdf
Graziano MU, Graziano KU, Pinto FMG, Bruna CQ de M, Souza RQ de, Lascala CA. Eficacia de la desinfección con alcohol al 70% (p/v) de superficies contaminadas sin limpieza previa. Rev Lat Am Enfermagem. 2013;21(2):618-23. DOI: https://doi.org/10.1590/S0104-11692013000200020
Badanian A. Bioseguridad en odontología en tiempos de pandemia COVID-19. Odontoestomatologia. 2020;22(35):1–20. DOI: https://doi.org/10.22592/ode2020nespa2
León Molina J, Abad-Corpa E. Desinfectantes y antisépticos frente al coronavirus: Síntesis de evidencias y recomendaciones. Enferm Clin. 2021;31:S84-8. DOI: https://doi.org/10.1016/j.enfcli.2020.05.013
Gamboa AVI. Comparación del efecto desinfectante entre lysol y eucida en las superficies de las jeringas triples de las Unidades Odontológicas de la Clínica Integral de séptimo semestre de la Facultad de Odontología de la Universidad Central del Ecuador. [Internet] [Tesis de Pregrado]. Universidad Central del Ecuador; 2015. Disponible en: http://www.dspace.uce.edu.ec/bitstream/25000/4052/1/T-UCE-0015-143.pdf
Benckiser R. Lysol® Disinfectant Spray-All Scents FICHA DE DATOS DE SEGURIDAD. Health Hygiene [Internet]. 2019. Disponible en: https://www.rbnainfo.com/MSDS/US/LYSOL Disinfectant Spray - All Scents SP GHS.pdf
Maeso G, Cano C. Desinfectantes en la clínica dental. Gd [Internet]. 2018;305:134–48. Disponible en: https://www.gacetadental.com/wp-content/uploads/2018/09/305_INFORME_Desinfectantes.pdf
Murcia JA, Callejas JA. Evaluación del desempeño de un lodo de perforación base aceite a escala de laboratorio implementando arcilla organofílica modificada [Internet] [Tesis de Pregrado]. Universidad de América; 2021. Disponible en: http://52.0.229.99/bitstream/20.500.11839/8630/1/5152310-2021-2-IP.pdf
Gerba CP. Quaternary ammonium biocides: Efficacy in application. Appl Environ Microbiol. 2015;81(2):464-9. DOI: https://doi.org/10.1128/AEM.02633-14
BD. Fluid thioglycollate medium. [Internet]. 2015. Disponible en: https://www.bd.com/resource.aspx?IDX=8459
Lewis JS, Weinstein MP, Bobenchik AM, Campeau S, Cullen SK, Galas MF, et al. CLSI M100-ED32:2022 Performance Standards for Antimicrobial Susceptibility Testing. [Internet]. 32nd Editi. Clinical and Laboratory Standards Institute - CLSI; 2022.
Difco. Plate count agar/Standard methods agar. Becton Dickinson [Internet]. 2022. Disponible en: https://legacy.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/290005.pdf
Zaragoza MT, Sanchez Figueroa AS. Comparación De Diferentes Soluciones Antimicrobianas En La Desinfección Del Respaldo Del Sillón Dental. Odontologia Actual. 2014;137(1):12
Sheldon AT. Antiseptic “resistance”: Real or perceived threat? Clinical Infectious Diseases. 2005;40(11):1650-6. DOI: https://doi.org/10.1086/430063
Molinari JA, Nelson P. Cleaning and disinfection Investigation: Clorox healthcare ® hydrogen peroxide cleaner disinfectant wipes. The dental advisor [Internet]. 2015;(65):1–5. Disponible en: https://www.cloroxpro.ca/wp-content/uploads/2018/09/Cleaning-and-Disinfection-Investigation_cleaning-and-disinfection-investigation-clorox-healthcare-hydrogen-peroxide-cleaner-disinfectant-wipes.pdf
Mejías DLM. Comparación del efecto desinfectante entre Lysol IC y Benzaldina en dos superficies de los sillones dentales del área de periodoncia de la clínica odontológica Dr. Renè Puig Bentz de la Universidad Nacional Pedro Henríquez Ureña, período septiembre – diciembre 2019 [Internet] [Tesis de Pregrado]. Universidad Nacional Pedro Henríquez Ureña; 2019. Disponible en: https://repositorio.unphu.edu.do/bitstream/handle/123456789/2331/Comparaci%C3%B3n%20del%20efecto%20desinfectante%20entre%20Lysol%20IC%20y%20Benzaldina%20en%20dos%20superficies.pdf?sequence=1&isAllowed=y
Zambrano-Gari Camilo C, Luna-Fontalvo Alberto J. Diversidad microbiana presente en el ambiente de la Clínica Odontológica de la Universidad del Magdalena. Rev Intropica. 2013;8:61-8.
Palenik CJ. The effect of long-term disinfection on clinical contact surfaces. JADA. 2012;143(5):472–7. DOI: https://doi.org/10.14219/jada.archive.2012.0207
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Camila Carvajal, Boris Villacres, Juan Carvajal, Maria Cristina Rockenbach
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution 4.0 International License.
AUTHORS RETAIN THEIR RIGHTS:
a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.
b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Odontología Sanmarquina.
c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).