Introduction and multiplication in vitro of garlic culture var. Morado Barranquino

Authors

  • Karina Carhuaricra E. Universidad Nacional Daniel Alcides Carrión - Facultad de Ciencias Agropecuarias. Ciudad Universitaria km 3 Carretera Cen-tral Miraflores – Oxapampa
  • Julio Olivera S. Estación Experimental Agraria DONOSO -Instituto Nacional de Innovación Agraria. Altura km 5,4 de la Carretera Chancay – Huaral
  • Javier Gonzales A. Universidad Nacional Daniel Alcides Carrión - Facultad de Ciencias Agropecuarias. Ciudad Universitaria km 3 Carretera Cen-tral Miraflores – Oxapampa
  • Juan Rodríguez L. Universidad Nacional Daniel Alcides Carrión - Facultad de Ciencias Agropecuarias. Ciudad Universitaria km 3 Carretera Cen-tral Miraflores – Oxapampa

DOI:

https://doi.org/10.15381/rpb.v19i3.1051

Keywords:

Allium sativum, explants, in vitro, meristem caulinar, subcultive

Abstract

We determine the effect of modified culture medium using different growth regulators concentrations (auxins / cytokinins) for regeneration and propagation of in vitro garlic plants (Allium sativumL.) var. ‘Morado Barrran-quino’. In the introduction stage meristem tips were cultured in test tubes up to regeneration. It was determined that medium MSM + IBA 0.6 mg/L was most suitable for the meristem tip differentiation and development. 13 weeks old microplants obtained on introduction stage were used for multiplication, obtaining the formation of a cluster after each subcultive. The best multiplication ratio was obtained in MMA medium + IAA 2.5 mg/L + KIN 5.0 mg/L and MSM medium + 2iP 1.5 mg/L with 4.71 and 4.79 respectively.

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Published

12/31/2012

Issue

Vol. 19 No. 3 (2012)

Section

Notes

License

Copyright (c) 2012 Karina Carhuaricra E., Julio Olivera S., Javier Gonzales A., Juan Rodríguez L.

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How to Cite

Carhuaricra E., Karina, Julio Olivera S., Javier Gonzales A., and Juan Rodríguez L. 2012. “Introduction and Multiplication in Vitro of Garlic Culture Var. Morado Barranquino”. Revista Peruana De Biología 19 (3): 341-44. https://doi.org/10.15381/rpb.v19i3.1051.