In vitro multiplication and somatic embryogenesis of Perezia pinnatifida (Asteraceae) medicinal Andean plant

Authors

  • Percy Olivera-Gonzales Laboratorio de Biología, Universidad Nacional Santiago Antúnez de Mayolo (UNASAM), Av. Centenario 200, Huaraz - Ancash, Perú https://orcid.org/0000-0002-8263-5901
  • Rosa Espinoza del Río Laboratorio de Biología, Universidad Nacional Santiago Antúnez de Mayolo (UNASAM), Av. Centenario 200, Huaraz - Ancash, Perú
  • Carmen Tamariz-Angeles Laboratorio de Biología, Universidad Nacional Santiago Antúnez de Mayolo (UNASAM), Av. Centenario 200, Huaraz - Ancash, Perú https://orcid.org/0000-0001-5252-2757

DOI:

https://doi.org/10.15381/rpb.v24i3.13911

Keywords:

Medicinal plant, Micropropagation, Somatic embryo, Plant tissue culture, clonal propagation

Abstract

This work inform on in vitro propagation of the "valeriana" Perezia pinnatifida (Humb. & Bonpl.) Wedd. Shoot multiplication and indirect somatic embryogenesis methodologies were performed. The basal culture medium for all stages was Murashige and Skoog, middle of salts supplemented with 2.0% sucrose, 0.3% phytagel and pH 5.67; the treatments were prepared with or without phytohormones. The hormonal supplements for the shoot multiplication were: BAP 1.0 mg.L-1 + ANA 0.01 mg.L-1, and BAP 1.0 mg.L-1; for embryogenic callus induction were: ANA or 2,4-D (1.0 mg.L-1 and 2.0 mg.L-1); and for the embryo germination were: BAP (0.5 and 1.0 mg.L-1) or BAP 0.5mg.L-1 + ANA 0.05mg.L-1. BAP 1.0 mg.L-1 produced the higher number buds. For somatic embryogenesis, ANA 1.0 mg.L-1 induced a greater area of embryogenic callus, and BAP 0.5 mg.L-1 allowed major germination of the somatic embryos.

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Published

10/28/2017

Issue

Vol. 24 No. 3 (2017)

Section

Notes

License

Copyright (c) 2017 Percy Olivera-Gonzales, Rosa Espinoza del Río, Carmen Tamariz-Angeles

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista Peruana de Biologia.

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating its initial publication in the Revista Peruana de Biologia (the originator of the work, journal, volume, number and date).

How to Cite

Olivera-Gonzales, Percy, Rosa Espinoza del Río, and Carmen Tamariz-Angeles. 2017. “In Vitro Multiplication and Somatic Embryogenesis of Perezia Pinnatifida (Asteraceae) Medicinal Andean Plant”. Revista Peruana De Biología 24 (3): 323-28. https://doi.org/10.15381/rpb.v24i3.13911.