Differential gene expression of some lignocellulolytic enzymes in Aspergillus niger biofilms

Authors

  • Gretty K. Villena Laboratorio de Micología y Biotecnología, Universidad Nacional Agraria La Molina, Lima, Perú
  • T. Fujikawa Institute for Molecular Biology and Biotechnology, Shizuoka University, Shizuoka, Japan.
  • S. Tsuyumu Institute for Molecular Biology and Biotechnology, Shizuoka University, Shizuoka, Japan.
  • Marcel Gutiérrez Correa Laboratorio de Micología y Biotecnología, Universidad Nacional Agraria La Molina, Lima, Perú

DOI:

https://doi.org/10.15381/rpb.v15i2.1731

Keywords:

Aspergillus niger, biofilms, gene expression, cellulases, xylanases

Abstract

A preliminary evaluation of transcriptional gene expression in Aspergillus niger ATCC 10864 biofilms developed on polyester cloth was carried out. The expression analysis of genes encoding some lignocellulolytic enzymes and some regulatory genes by means of RT-PCR showed that eng1, eglC, exo, eglA, eglB and xynB genes are differentially expressed in biofilm fermentation either time-related or through the production of more than a transcript as compared to A. niger grown in submerged fermentation. Likewise, the regulatory genes xlnR and creA showed time-related expression patterns that were different in both fermentation systems. Results attained in this work contribute with an initial molecular evidence of differential gene expression as well as differential gene regulation patterns in fungal biofilms that may be related to cell adhesion.

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Published

08/18/2008

Issue

Vol. 15 No. 2 (2008)

Section

Articles

License

Copyright (c) 2008 Gretty K. Villena, T. Fujikawa, S. Tsuyumu, Marcel Gutiérrez Correa

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How to Cite

Villena, Gretty K., T. Fujikawa, S. Tsuyumu, and Marcel Gutiérrez Correa. 2008. “Differential Gene Expression of Some Lignocellulolytic Enzymes in Aspergillus Niger Biofilms”. Revista Peruana De Biología 15 (2): 97-102. https://doi.org/10.15381/rpb.v15i2.1731.