Toxic activity of Argemone subfusiformis Ownb. and Tagetes patula Link against Aedes aegypti L. fourth instar larvae and pupae

Abstract

The aim of this research work was evaluate toxic activity of ethanolic extracts from Argemone subfusiformis “Holy thistle” & Tagetes patula “French marigold” leaves against Aedes aegypti fourth instar larvae and pupae were evaluated. Bioassay and extract processing were performed in Entomology Laboratory of National University of Trujillo, Perú, from April to December, 2007, outlined by World Health Organization (2005) standard protocol. Larvae and pupae mortality rates reached using A. subfusiformis extract were 100% with concentrations of 76,8 mg/L. and 153,6 mg/L. at 12 hours of exposure, while pupae mortality was to a concentration of 153,6 mg/L. at 24 hours of exposure. By using T. patula extract mortality rates reached were 92% and 77% on larvae and pupae, respectively with a concentration of 153,6 mg/L. at 48 hours of exposure. A. subfusiformis extracts showed LC50 and LC90 values at 48 hours on larvae which were 6,24 mg/L. and 9,91 mg/L. and pupae LC50 and LC90 values were 9,45 mg/L. and 16,92 mg/L. T patula extracts showed LC50 and LC90 values at 48 hours on larvae which were 72,21 mg/L. and 137,37 mg/L. and pupae LC50 and LC90 values were 89,1 mg/L. and 167,38 mg/L. According to ANAVA were observed significant difference among four times of exposure and five concentration levels. Larvae and pupae susceptibilities were assessed by means of log-dosage/probit lines. Both species showed leaf toxic activities against A. aegypti fourth instar larvae and pupae, being the major values of mortality to A. subfusiformis.