Production of lactic acid by Lactobacillus plantarum L10 on batch and continuous cultivation

Authors

  • Waldir Estela Universidad de Tecnología Química, Praga, Departamento de Fermentación Química y Bioingeniería, Praga, República Checa.Universidad Nacional Micaela Bastidas de Apurimac, Facultad de Ingeniería Agroindustrial, Laboratório de Biotecnologia Agroindustrial.
  • Mojmír Rychtera Universidad de Tecnología Química, Praga, Departamento de Fermentación Química y Bioingeniería, Praga, República Checa.
  • Karel Melzoch Universidad de Tecnología Química, Praga, Departamento de Fermentación Química y Bioingeniería, Praga, República Checa.
  • Elena Quillama Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas. Laboratorio de Microbiología Industrial y Biotecnología Alimentaria. Apartado postal 110058, Lima11, Perú.
  • Erida Egoavil Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas. Laboratorio de Microbiología Industrial y Biotecnología Alimentaria. Apartado postal 110058, Lima11, Perú.

DOI:

https://doi.org/10.15381/rpb.v14i2.1797

Keywords:

Lactic bacteria, Lactobacillus plantarum, batch cultivation, continuous cultivation, chemostat, lactic acid.

Abstract

Strain of Lactobacillus plantarum L10 was tested at laboratory scale, in order to evaluate the production of lactic acid on batch and continuous cultivation. Furthermore, optimization of culture medium and cultivation conditions were carried out too. The best parameters obtained for lactic acid production on batch cultivation were: YP/S 86,1%; PP 5,4 g/L/h; joined to YX/S 13,2%; PX 1,2 g/L/h and μ = 0,2 h-1, the medium composition was as follows: glucose 70 g/L; yeast extract 12,1 g/L; KH2PO4 1,2 g/L; (NH4)2HPO4 1,2 g/L; ammonium citrate 3,0 g/L; MgSO4. 7H2O 0,3 g/L and MnSO4. 4H2O 0,03 g/L. Respecting to continuous cultivation the best parameters obtained for production of lactic acid were: YP/S 96%; P´P 6,0 g/L/h; joined to YX/S 19%; P´X 1,2 g/L/h; and dilution rate (D) 0,46 h-1.

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Published

12/31/2007

Issue

Vol. 14 No. 2 (2007)

Section

Articles

License

Copyright (c) 2007 Waldir Estela, Mojmír Rychtera, Karel Melzoch, Elena Quillama, Erida Egoavil

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How to Cite

Estela, Waldir, Mojmír Rychtera, Karel Melzoch, Elena Quillama, and Erida Egoavil. 2007. “Production of Lactic Acid by Lactobacillus Plantarum L10 on Batch and Continuous Cultivation”. Revista Peruana De Biología 14 (2): 271-75. https://doi.org/10.15381/rpb.v14i2.1797.