In vitro conservation of Dioscorea alata L. clon caraqueño (Dioscoreaceae)

Authors

  • Misterbino Borges García Centro de Estudios de Biotecnología Vegetal, Facultad de Ciencias Agrícolas, Universidad de Granma, Bayamo 85100 Granma, Cuba.
  • Yaimara Alarcón Sánchez Centro de Estudios de Biotecnología Vegetal, Facultad de Ciencias Agrícolas, Universidad de Granma, Bayamo 85100 Granma, Cuba.
  • Bernard Malaurie Institut de Recherche pour le Développement (IRD), UMR DIAPC, F-34 000 Montpellier, France.
  • Yanet Hernandez Jerez Centro de Estudios de Biotecnología Vegetal, Facultad de Ciencias Agrícolas, Universidad de Granma, Bayamo 85100 Granma, Cuba.
  • Juan Jose Silva Pupo Centro de Estudios de Biotecnología Vegetal, Facultad de Ciencias Agrícolas, Universidad de Granma, Bayamo 85100 Granma, Cuba.

DOI:

https://doi.org/10.15381/rpb.v16i2.207

Keywords:

yam, medium-term conservation, cultivation in vitro, mannitol, BAP.

Abstract

In this study, we report an efficient method for conservation in vitro of Dioscorea alata L. clone caraqueño during 9 and 12 months based on the culture medium modification (MS to 75% + vitamins MS + sucrose 30 g.L-1 + activated charcoal 2 g.L-1) with different manitol (0; 0,5 and 1,5%) and BAP (0; 0,1 mg.L-1) levels. At 9 and 12 months of in vitro conservation, the following evaluations were determined: survival (%), leaf senility (%), shoot length, buds and microtuber explant count. Plant regeneration (%), shoot length, leaf number and bud explant count were determined at 8 weeks of the nodal cutting regeneration (from vitroplants conserved for 9 and 12 months) in the culture medium (MS to 75% + vitamins MS + sucrose 30 g.L-1 + activated charcoal 2 g.L-1). .Shoot length, leaf number and bud explant count were evaluated at 5 weeks during the conventional micropropagation. The cultivation variants formed by media MS to 75% + vitamins MS + sucrose 30 g.L-1 + activated charcoal 2 g.L-1 and MS to 75% + vitamins MS + sucrose 30 g. l-1 + activated charcoal 2 g.L-1 + BAP 0, 1 mg.L-1 allowed an effective way the conservation of in vitro plants from nodal cutting of clone caraqueño yam during 9 and 12 months with high survival percentages, a significant number of microtubers, smallest leaf senility percentages and 100% regeneration in whole plants with a normal growth in micropropagation conditions.

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Published

12/31/2009

Issue

Vol. 16 No. 2 (2009)

Section

Articles

License

Copyright (c) 2009 Misterbino Borges García, Yaimara Alarcón Sánchez, Bernard Malaurie, Yanet Hernandez Jerez, Juan Jose Silva Pupo

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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How to Cite

Borges García, Misterbino, Yaimara Alarcón Sánchez, Bernard Malaurie, Yanet Hernandez Jerez, and Juan Jose Silva Pupo. 2009. “In Vitro Conservation of Dioscorea Alata L. Clon caraqueño (Dioscoreaceae)”. Revista Peruana De Biología 16 (2): 203-8. https://doi.org/10.15381/rpb.v16i2.207.