Partial purification and characterization of an antimicrobial substance produced by a marine Alteromonas sp.

Authors

  • Jorge León Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos- Lima , Perú
  • Guillermina Tapia Laboratorio de Bioquímica, Facultad de Ciencias Básicas – Carrera de Bioquímica – Universidad Católica de Valparaíso, Chile
  • Rita Avalos Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos- Lima , Perú

DOI:

https://doi.org/10.15381/rpb.v12i3.2411

Keywords:

marine bacteria, inhibitory substances, extracellular substance, Alteromonas, antibiosis

Abstract

A member of the genus Alteromonas sp. (strain N22.C) isolated from marine neuston and substance-producer which inhibit growth of ichthyopathogenics was characterized phenotipically by means of morphological, physiological and biochemical conventional tests. To determine the chemical nature of the inhibitory substance, raw extracts of supernatans from cultured strains were precipitated with increasing concentrations of Ammonium Sulphate up to 70% and filtered through columns of Sephadex G-25. Assays by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS–PAGE) revealed that the antimicrobial substance is a proteinaceous compound with a molecular mass of approximately 34000 Da, which lacks sugar associated residues of it. Other assays made with raw semipurified fractions extracts of Alteromonas sp N22.C show the wide spectrum of antibiotic activity against pathogenic bacterial strains of fish, mollusks and crustaceans.

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Published

12/30/2005

Issue

Vol. 12 No. 3 (2005)

Section

Articles

License

Copyright (c) 2005 Jorge León, Guillermina Tapia, Rita Avalos

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating its initial publication in the Revista Peruana de Biologia (the originator of the work, journal, volume, number and date).

How to Cite

León, Jorge, Guillermina Tapia, and Rita Avalos. 2005. “Partial Purification and Characterization of an Antimicrobial Substance Produced by a Marine Alteromonas Sp”. Revista Peruana De Biología 12 (3): 359-68. https://doi.org/10.15381/rpb.v12i3.2411.