Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)

Authors

  • Sergio Huatuco Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos
  • Enrique Escobar Laboratorio de Bioquímica y Genética Molecular. Facultad de Ciencias Biológicas
  • Armando Yarlequé Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos

DOI:

https://doi.org/10.15381/rpb.v11i1.2436

Keywords:

myotoxin, Bothrops atrox, snake venom, myonecrosis

Abstract

A myotoxin from Bothrops atrox snake venom was purified by cationic exchange on CM-Sephadex C-50 with 0,05M ammonium acetate pH 7. The myotoxin is a basic protein and by gel filtration and PAGE-SDS was demonstrated that protein has a molecular weight of 27 kDa and two polipeptides chain of 14 kDa each one. The inoculation of myotoxin in gastrocnemius muscle of white mice produce liberation of creatin kinase as well as myonecrosis. The myotoxin has phospholipasic, anticoagulant and edematic activity, but not hemolytic activity.

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Published

07/15/2004

Issue

Vol. 11 No. 1 (2004)

Section

Articles

License

Copyright (c) 2004 Sergio Huatuco, Enrique Escobar, Armando Yarlequé

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How to Cite

Huatuco, Sergio, Enrique Escobar, and Armando Yarlequé. 2004. “Isolation and Partial Characterization of a Myotoxin from Bothrops Atrox Snake Venom (Ophidia: Viperidae)”. Revista Peruana De Biología 11 (1): 79-86. https://doi.org/10.15381/rpb.v11i1.2436.