Intraluminar testicular colonization and differentiation of the inner cell mass in mice (Mus Musculus)

Authors

  • Láyonal Acosta Biología del Desarrollo, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Casilla 11-0058, Lima 11, Perú. Tel.: +51 6 197000 – 1529; fax: +51 6 197000 – 1509.
  • Víctor Núñez Biología del Desarrollo, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Casilla 11-0058, Lima 11, Perú. Tel.: +51 6 197000 – 1529; fax: +51 6 197000 – 1509.
  • Jose Pino Biología del Desarrollo, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Casilla 11-0058, Lima 11, Perú. Tel.: +51 6 197000 – 1529; fax: +51 6 197000 – 1509.
  • Betty Shiga Biología del Desarrollo, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Casilla 11-0058, Lima 11, Perú. Tel.: +51 6 197000 – 1529; fax: +51 6 197000 – 1509.

DOI:

https://doi.org/10.15381/rpb.v18i3.454

Keywords:

Pluripotent stem cells, germ stem cells, testes, minitubules, spermatogenesis.

Abstract

Primordial germ cells (PGC`s) are transplanted to testicle of other individual of the same species, they colonize the lumen of the seminiferous tubules, seeking a niche to differentiate into sperm. Our objective was to evaluate the intraluminal colonization of a suspension of cells in the inner cell mass (IMC`s) of blastocysts obtained from mice, using a novel technique. It was transplanted a suspension of ICM by mean of inmunosurgery into the rete testis of recipient animals which were previously treated with cyclophosphamide to reduce their own spermatogenesis. We confirmed the presence of intraluminal minitubules in 2 of 100 seminiferous tubules, demonstrating that transplantation of a suspension of cells from the inner cell mass can colonize the seminiferous tubules and also maintain a synchronously xenogenic spermatogenesis with the receiver.

Downloads

Download data is not yet available.

Downloads

Published

12/30/2011

Issue

Vol. 18 No. 3 (2011)

Section

Articles

License

Copyright (c) 2011 Láyonal Acosta, Víctor Núñez, Jose Pino, Betty Shiga

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

a. Authors retain their trade mark rights and patent, and also on any process or procedure described in the article.

b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista Peruana de Biologia.

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating its initial publication in the Revista Peruana de Biologia (the originator of the work, journal, volume, number and date).

How to Cite

Acosta, Láyonal, Víctor Núñez, Jose Pino, and Betty Shiga. 2011. “Intraluminar Testicular Colonization and Differentiation of the Inner Cell Mass in Mice (Mus Musculus)”. Revista Peruana De Biología 18 (3): 367-72. https://doi.org/10.15381/rpb.v18i3.454.