Identification of genes related to drought in native potatoes using RNA-Seq

Authors

  • Yerisf Torres Unidad de genómica, Laboratorios de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia. Av. Honorio Delgado 430, Urb. Ingeniería, S.M.P. Lima - Perú.
  • Roberto Lozano Unidad de genómica, Laboratorios de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia. Av. Honorio Delgado 430, Urb. Ingeniería, S.M.P. Lima - Perú.
  • Carlos Merino Unidad de genómica, Laboratorios de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia. Av. Honorio Delgado 430, Urb. Ingeniería, S.M.P. Lima - Perú.
  • Gisella Orjeda Unidad de genómica, Laboratorios de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia. Av. Honorio Delgado 430, Urb. Ingeniería, S.M.P. Lima - Perú.

DOI:

https://doi.org/10.15381/rpb.v20i3.5208

Keywords:

RNA-Seq, transcriptome, drought, potato, mRNA.

Abstract

The recent advent RNA sequencing technology (RNA-Seq), a massively parallel sequencing method for transcriptome analysis, provides an opportunity to understand the expression profile of plants in response to biotic and abiotic stress. In this study, the mRNA was sequencing from leaves and roots of two native potato varieties at different levels of drought. Fifty-base-pair reads from whole mRNAs were mapped to the potato genomic sequence: 75 – 82% mapped uniquely to the genome, 6 – 14% mapped to several locations in the genome and 9 – 12% had no match in the genome. Comparing expression profiles, 887 to 1925 genes were found to be induced/repressed by drought in the sensible variety and 998 to 1995 in the tolerant. This research provides valuable information for future studies and deeper understanding of the molecular mechanism of drought resistance in potato and related species.

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Published

12/30/2013

Issue

Vol. 20 No. 3 (2013)

Section

Articles

License

Copyright (c) 2013 Yerisf Torres, Roberto Lozano, Carlos Merino, Gisella Orjeda

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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b. Authors retain their right to share, copy, distribute, perform and publicly communicate their article (eg, to place their article in an institutional repository or publish it in a book), with an acknowledgment of its initial publication in the Revista Peruana de Biologia.

c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating its initial publication in the Revista Peruana de Biologia (the originator of the work, journal, volume, number and date).

How to Cite

Torres, Yerisf, Roberto Lozano, Carlos Merino, and Gisella Orjeda. 2013. “Identification of Genes Related to Drought in Native Potatoes Using RNA-Seq”. Revista Peruana De Biología 20 (3): 211-14. https://doi.org/10.15381/rpb.v20i3.5208.