The thiocyanate degradation by fungi isolated from mining environments and evaluation of degradative capacity

Authors

  • Susan Medina 1Laboratorio de Microbiología Molecular y Biotecnología, 2Laboratorio de Sistemática Molecular y Filogeografía. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Avenida Venezuela s/n, Lima 1, Perú.
  • Marisela Torres 1Laboratorio de Microbiología Molecular y Biotecnología, 2Laboratorio de Sistemática Molecular y Filogeografía. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Avenida Venezuela s/n, Lima 1, Perú.
  • Yerson Durán 1Laboratorio de Microbiología Molecular y Biotecnología, 2Laboratorio de Sistemática Molecular y Filogeografía. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Avenida Venezuela s/n, Lima 1, Perú.
  • Rina Ramírez 1Laboratorio de Microbiología Molecular y Biotecnología, 2Laboratorio de Sistemática Molecular y Filogeografía. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Avenida Venezuela s/n, Lima 1, Perú.
  • Juan Herrera 1Laboratorio de Microbiología Molecular y Biotecnología, 2Laboratorio de Sistemática Molecular y Filogeografía. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Avenida Venezuela s/n, Lima 1, Perú.
  • Pablo Ramírez 1Laboratorio de Microbiología Molecular y Biotecnología, 2Laboratorio de Sistemática Molecular y Filogeografía. Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Avenida Venezuela s/n, Lima 1, Perú.

DOI:

https://doi.org/10.15381/rpb.v19i1.791

Keywords:

thiocyanate, fungus, Fusarium trincictum, degradation, mining.

Abstract

used in gold production this compound is very toxic especially for environment, by nature reaction with sulfur it converts into thiocyanate (-SCN). In this paper, we isolate -SCN degrading fungus from Junin and Tumbes mining zones. Strains were assayed on 5 to 600 mM -SCN concentration, at least thiocyanate degrading kinetic was assayed at 1.2 g.L-1 of KSCN initial concentration, then the best strains had been morphological, biochemical and molecular identified using ITS RNA molecular marker. From 58 isolated fungus we found 4 unparfait funguses with a great thiocyanate degrading capacity those strains could be used for bioremediation processes. Thiocyanate degrading capacity of these strains was 50 g.L-1 on plate into 72 hours and their average capacity was 10.05 mg.L-1 -SCN with an standard velocity of -SCN degradation of 28.77 mg.L-1.h-1. Two strains were molecular identified as Fusarium trincictum by using ITS rDNA gene.

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Published

10/02/2012

Issue

Vol. 19 No. 1 (2012)

Section

Articles

License

Copyright (c) 2012 Susan Medina, Marisela Torres, Yerson Durán, Rina Ramírez, Juan Herrera, Pablo Ramírez

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How to Cite

Medina, Susan, Marisela Torres, Yerson Durán, Rina Ramírez, Juan Herrera, and Pablo Ramírez. 2012. “The Thiocyanate Degradation by Fungi Isolated from Mining Environments and Evaluation of Degradative Capacity”. Revista Peruana De Biología 19 (1): 081-088. https://doi.org/10.15381/rpb.v19i1.791.