In vitro antimicrobial activity of 3MIX-MP and CTZ pastes against Enterococcus faecalis ATCC® 29212

Authors

DOI:

https://doi.org/10.15381/os.v26i1.23182

Keywords:

Antimicrobials, Pulpotomy, Enterococcus faecalis, Pediatric dentistry

Abstract

Objective. To determine the antibacterial effect of triantibiotic paste (3MIX-MP) and chloramphenicol-tetracycline-ZOE (CTZ) antibiotic paste at 24, 48, 72 hours and 7 days on Enterococcus faecalis. Methods. Experimental, in vitro, comparative study, with a non-probabilistic sample for convenience of 210 paper discs, distributed in 30 Petri dishes previously prepared with Mitis Salivarius agar and Mueller Hinton agar and inoculated with strains of Enterococcus faecalis ATCC® 29212. The method was used. Kirby-Bauer, inoculated with a 0.5 McFarland suspension of Enterococcus faecalis. Filter paper discs were placed inside the wells and 10 µl of antimicrobial pastes and control were supplied. The positive control was zinc oxide-eugenol cement discs; halo diameters were measured with a vernier caliper. A repeated measures ANOVA was used for intragroup comparisons, one-way ANOVA for intergroup comparisons, and Tukey’s post hoc tests. Results. After 24 hours, the 3MIX-MP paste obtained larger inhibition zone diameters than the CTZ paste with an average of 39,24 mm and 34,19 mm, respectively, and a minimum of 36,26 mm and 26,38 mm at 24 hours. 7 days. Statistically significant differences were observed between the halos obtained from the 3MIX-MP paste and the CTZ paste, except for the control after 48 hours. Conclusions. It was determined that the 3MIX-MP paste has a greater inhibitory action in vitro compared to the CTZ paste, the diameter of the inhibitory halos decreased as the exposure time to the microorganism increased.

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Author Biographies

  • Jesús Antonio Vargas-Mendoza, Universidad Nacional del Altiplano, Puno, Peru

    Dental surgeon, born in the city of Arequipa in Peru. Higher studies completed at the National University of the Altiplano of Puno. Destined to grow as a person and as a health professional to provide adequate comprehensive care both in the workplace and in the social and personal spheres. Having as a reference and base, the mission and vision instilled by the higher house of Dentistry studies of the aforementioned university.

  • Rosini Alicia Mamani-Quispe, Universidad Nacional del Altiplano, Puno, Peru

    Formación academica Pregrado en Universidad Nacional del Altiplano - Puno - Perú, Internado Hospitalario en Hospital Militar - División del ejercito III Arequipa, Cirujano Dentista certificada por el Colegio Odontológico del Perú. staff de Clínica Dental Protecdent y Centro Odontológico Perú.

  • Jorge Luis Mercado-Portal, Universidad Nacional del Altiplano, Puno, Peru

    Surgeon-Dentist, from the Universidad Mayor de San Andres -Bolivia, specializing in Periodontics, Magister in Stomatology Universidad Peruana Cayetano Heredia; Doctor of Science: Public Health, National University of San Agustín de Arequipa, more than 20 years of experience in undergraduate and post-graduate university teaching in the area of Public Health and Research - National University of the Altiplano, currently Dean of the Faculty of health sciences, clinical experience in the area of Periodontics, with a scientific production of books and publication of scientific articles in indexed journals; Advisor Director of undergraduate and postgraduate research projects, participant in numerous specialized academic events on research, periodontics, forensic and legal topics.

  • Abel Josue Aguilar-Vilca, Universidad Nacional del Altiplano, Puno, Peru

    Undergraduate student at the Professional School of Dentistry, Faculty of Health Sciences of the National University of the Altiplano.

  • Tania Carola Padilla-Cáceres, Universidad Nacional del Altiplano, Puno, Peru

    Doctor in Health Sciences and Specialist in pediatric dentistry, orthodontics and maxillary orthopedics. She is a dental surgeon certified by the Dental College of Peru, belonging to the fifth superior of the promotion. Member of the year 2008 awarded by the Peruvian Society of Pediatric Dentistry. Excellence Award 2013 awarded by the National University of the Altiplano. Principal Professor, Undergraduate and Postgraduate teacher at the National University of the Altiplano Puno. Corresponding member of the advisory council of the Hipólito Unanue Institute Foundation. Volunteer at Operation Smile Peru. Qualified as researcher CONCYTEC Carlos Monge IV. Author of scientific articles in national and international journals. Lecturer at national and international courses and conferences.

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Published

2023-01-03

Issue

Vol. 26 No. 1 (2023)

Section

Articles

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Copyright (c) 2023 Jesús Antonio Vargas-Mendoza, Rosini Alicia Mamani-Quispe, Jorge Luis Mercado-Portal, Abel Josue Aguilar-Vilca, Tania Carola Padilla-Cáceres

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How to Cite

1.
Vargas-Mendoza JA, Mamani-Quispe RA, Mercado-Portal JL, Aguilar-Vilca AJ, Padilla-Cáceres TC. In vitro antimicrobial activity of 3MIX-MP and CTZ pastes against Enterococcus faecalis ATCC® 29212. Odontol Sanmarquina [Internet]. 2023 Jan. 3 [cited 2024 Jul. 17];26(1):e23182. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/23182