Analysis of the distribution of microhardness, mineral, and organic content of human, bovine, and ovine teeth

Authors

  • Felipe Weidenbach Degrazia School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil https://orcid.org/0000-0002-2350-5293
  • Jaqueline Wermeier Rippel Dental Materials Department, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
  • Isadora Martini Garcia Department of General Dentistry, Division of Operative Dentistry, University of Maryland School of Dentistry, Baltimore, MD, USA
  • Bruna Genari School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil https://orcid.org/0000-0002-6329-9543
  • Susana Maria Werner Samuel Dental Materials Department, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
  • Fabrício Mezzomo Collares Dental Materials Department, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-1382-0150

DOI:

https://doi.org/10.15381/os.v26i3.24730

Keywords:

Spectrum Analysis, Raman, Hardness, Collagen Type I, Collagen Type III, Models, Animal

Abstract

Objetive. Human teeth have been commonly used for in vitro and in situ studies. Cu­rrently, other animals’ teeth have been purposed for dental research to overcome human teeth’ problematic availability. This study aimed to investigate the enamel and dentin from human, bovine, and ovine teeth concerning the microhardness, organic, and inorganic contents via micro-Raman spectroscopy. Methods. Human, bovine, and ovine teeth were divided according to their type and age into seven groups: Ovine; Bovine-12 months; Bovine-24 months; Bovine-36 months; Bovine-48 months; Bovine-+60 months; Human (control). The enamel’s microhardness (superficial and deep) and dentin (superficial, middle, and deep) were analyzed. The calcium/phosphate ratio and amide contents were determined by micro-Raman spectroscopy. Results. Overall, the microhardness of human enamel was superior to the other species. Dentin’s microhardness was similar among groups. Ovine group showed lower values of calcium/phosphate ratio than human. Amide content was similar between bovine and human. The microhardness and calcium/phosphate ratio of enamel and dentin, respectively, decreased as the age of bovine teeth increased. Conclusions. Researchers must be aware and take into consideration the differences of ovine and bovine enamel compared to human enamel. Other alternatives that are more similar to the microhardness of human enamel should be sought. Bovine teeth of 12 and 24 months are suitable substitutes for dentin of human teeth. Researchers must also be aware of the age of the animals and specify it in the studies.

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Published

2023-09-29

Issue

Vol. 26 No. 3 (2023)

Section

Articles

License

Copyright (c) 2023 Felipe Weidenbach Degrazia, Jaqueline Wermeier Rippel, Isadora Martini Garcia, Bruna Genari, Susana Maria Werner Samuel, Fabrício Mezzomo Collares

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This work is licensed under a Creative Commons Attribution 4.0 International License.

AUTHORS RETAIN THEIR RIGHTS:

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c. Authors retain theirs right to make a subsequent publication of their work, to use the article or any part thereof (eg a compilation of his papers, lecture notes, thesis, or a book), always indicating the source of publication (the originator of the work, journal, volume, number and date).

How to Cite

1.
Degrazia FW, Rippel JW, Garcia IM, Genari B, Werner Samuel SM, Mezzomo Collares F. Analysis of the distribution of microhardness, mineral, and organic content of human, bovine, and ovine teeth. Odontol Sanmarquina [Internet]. 2023 Sep. 29 [cited 2024 Jun. 13];26(3):e24730. Available from: https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/24730